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single particle cryo em approach  (Thermo Fisher)


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    Thermo Fisher single particle cryo em approach
    a–c <t>,</t> <t>Cryo-EM</t> images of the VLPs produced in the absence (a) or presence (b, c) of LEN. Scale bar, 100 nm. d , Cryo-EM density map of the CA lattice within the LEN-containing VLPs, shown from the top (left) and side (right) views. Scale bar, 100 Å. e , The atomic model of a CA hexamer in the LEN-bound lattice fitted into the cryo-EM density map shown in (d). Scale bar, 50 Å. f , Close-up view of the cryo-EM map of a LEN-bound CA lattice (d) and the atomic model (e) showing the density corresponding to a bound LEN. g , Close-up view of the cryo-ET map of a LEN-free CA lattice (EMDB: 13423) and its atomic model, showing the absence of density corresponding to the bound LEN. h , Comparison of two adjacent CA hexamers within the LEN-bound (blue) and LEN-free (yellow; EMDB: 13423) lattices, showing that LEN binding induces a flattening of the curvature between neighbouring hexamers.
    Single Particle Cryo Em Approach, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single particle cryo em approach/product/Thermo Fisher
    Average 99 stars, based on 14316 article reviews
    single particle cryo em approach - by Bioz Stars, 2026-04
    99/100 stars

    Images

    1) Product Images from "Structural basis of lenacapavir-induced HIV-1 capsid disruption during virion maturation"

    Article Title: Structural basis of lenacapavir-induced HIV-1 capsid disruption during virion maturation

    Journal: bioRxiv

    doi: 10.64898/2026.02.16.706074

    a–c , Cryo-EM images of the VLPs produced in the absence (a) or presence (b, c) of LEN. Scale bar, 100 nm. d , Cryo-EM density map of the CA lattice within the LEN-containing VLPs, shown from the top (left) and side (right) views. Scale bar, 100 Å. e , The atomic model of a CA hexamer in the LEN-bound lattice fitted into the cryo-EM density map shown in (d). Scale bar, 50 Å. f , Close-up view of the cryo-EM map of a LEN-bound CA lattice (d) and the atomic model (e) showing the density corresponding to a bound LEN. g , Close-up view of the cryo-ET map of a LEN-free CA lattice (EMDB: 13423) and its atomic model, showing the absence of density corresponding to the bound LEN. h , Comparison of two adjacent CA hexamers within the LEN-bound (blue) and LEN-free (yellow; EMDB: 13423) lattices, showing that LEN binding induces a flattening of the curvature between neighbouring hexamers.
    Figure Legend Snippet: a–c , Cryo-EM images of the VLPs produced in the absence (a) or presence (b, c) of LEN. Scale bar, 100 nm. d , Cryo-EM density map of the CA lattice within the LEN-containing VLPs, shown from the top (left) and side (right) views. Scale bar, 100 Å. e , The atomic model of a CA hexamer in the LEN-bound lattice fitted into the cryo-EM density map shown in (d). Scale bar, 50 Å. f , Close-up view of the cryo-EM map of a LEN-bound CA lattice (d) and the atomic model (e) showing the density corresponding to a bound LEN. g , Close-up view of the cryo-ET map of a LEN-free CA lattice (EMDB: 13423) and its atomic model, showing the absence of density corresponding to the bound LEN. h , Comparison of two adjacent CA hexamers within the LEN-bound (blue) and LEN-free (yellow; EMDB: 13423) lattices, showing that LEN binding induces a flattening of the curvature between neighbouring hexamers.

    Techniques Used: Cryo-EM Sample Prep, Produced, Tomography, Comparison, Binding Assay

    a , Overview of intra- and interhexamer interfaces within the HIV-1 capsid. b , Comparison of the CTD–CTD dimer interface between CA molecules mediated by helix 9, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the dimeric interface. Structures were superimposed by aligning one helix 9 of the CA-CTD of hexamer 1. c , Comparison of the CTD–CTD trimer interface between CA molecules mediated by primary helix 10, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 10 of the CA-CTD of hexamer 1. d , Comparison of the NTD–NTD interface between CA molecules mediated by helices 2 and 3, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 3 of the CA-NTD.
    Figure Legend Snippet: a , Overview of intra- and interhexamer interfaces within the HIV-1 capsid. b , Comparison of the CTD–CTD dimer interface between CA molecules mediated by helix 9, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the dimeric interface. Structures were superimposed by aligning one helix 9 of the CA-CTD of hexamer 1. c , Comparison of the CTD–CTD trimer interface between CA molecules mediated by primary helix 10, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 10 of the CA-CTD of hexamer 1. d , Comparison of the NTD–NTD interface between CA molecules mediated by helices 2 and 3, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 3 of the CA-NTD.

    Techniques Used: Comparison, Cryo-EM Sample Prep, Tomography



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    single particle cryo em approach  (Thermo Fisher)
    99
    Thermo Fisher single particle cryo em approach
    a–c <t>,</t> <t>Cryo-EM</t> images of the VLPs produced in the absence (a) or presence (b, c) of LEN. Scale bar, 100 nm. d , Cryo-EM density map of the CA lattice within the LEN-containing VLPs, shown from the top (left) and side (right) views. Scale bar, 100 Å. e , The atomic model of a CA hexamer in the LEN-bound lattice fitted into the cryo-EM density map shown in (d). Scale bar, 50 Å. f , Close-up view of the cryo-EM map of a LEN-bound CA lattice (d) and the atomic model (e) showing the density corresponding to a bound LEN. g , Close-up view of the cryo-ET map of a LEN-free CA lattice (EMDB: 13423) and its atomic model, showing the absence of density corresponding to the bound LEN. h , Comparison of two adjacent CA hexamers within the LEN-bound (blue) and LEN-free (yellow; EMDB: 13423) lattices, showing that LEN binding induces a flattening of the curvature between neighbouring hexamers.
    Single Particle Cryo Em Approach, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single particle cryo em approach/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    single particle cryo em approach - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    a–c , Cryo-EM images of the VLPs produced in the absence (a) or presence (b, c) of LEN. Scale bar, 100 nm. d , Cryo-EM density map of the CA lattice within the LEN-containing VLPs, shown from the top (left) and side (right) views. Scale bar, 100 Å. e , The atomic model of a CA hexamer in the LEN-bound lattice fitted into the cryo-EM density map shown in (d). Scale bar, 50 Å. f , Close-up view of the cryo-EM map of a LEN-bound CA lattice (d) and the atomic model (e) showing the density corresponding to a bound LEN. g , Close-up view of the cryo-ET map of a LEN-free CA lattice (EMDB: 13423) and its atomic model, showing the absence of density corresponding to the bound LEN. h , Comparison of two adjacent CA hexamers within the LEN-bound (blue) and LEN-free (yellow; EMDB: 13423) lattices, showing that LEN binding induces a flattening of the curvature between neighbouring hexamers.

    Journal: bioRxiv

    Article Title: Structural basis of lenacapavir-induced HIV-1 capsid disruption during virion maturation

    doi: 10.64898/2026.02.16.706074

    Figure Lengend Snippet: a–c , Cryo-EM images of the VLPs produced in the absence (a) or presence (b, c) of LEN. Scale bar, 100 nm. d , Cryo-EM density map of the CA lattice within the LEN-containing VLPs, shown from the top (left) and side (right) views. Scale bar, 100 Å. e , The atomic model of a CA hexamer in the LEN-bound lattice fitted into the cryo-EM density map shown in (d). Scale bar, 50 Å. f , Close-up view of the cryo-EM map of a LEN-bound CA lattice (d) and the atomic model (e) showing the density corresponding to a bound LEN. g , Close-up view of the cryo-ET map of a LEN-free CA lattice (EMDB: 13423) and its atomic model, showing the absence of density corresponding to the bound LEN. h , Comparison of two adjacent CA hexamers within the LEN-bound (blue) and LEN-free (yellow; EMDB: 13423) lattices, showing that LEN binding induces a flattening of the curvature between neighbouring hexamers.

    Article Snippet: We then determined the capsid lattice structure within VLPs produced in the presence of LEN using a single-particle cryo-EM approach ( , Figs. S2-3, Table S1).

    Techniques: Cryo-EM Sample Prep, Produced, Tomography, Comparison, Binding Assay

    a , Overview of intra- and interhexamer interfaces within the HIV-1 capsid. b , Comparison of the CTD–CTD dimer interface between CA molecules mediated by helix 9, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the dimeric interface. Structures were superimposed by aligning one helix 9 of the CA-CTD of hexamer 1. c , Comparison of the CTD–CTD trimer interface between CA molecules mediated by primary helix 10, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 10 of the CA-CTD of hexamer 1. d , Comparison of the NTD–NTD interface between CA molecules mediated by helices 2 and 3, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 3 of the CA-NTD.

    Journal: bioRxiv

    Article Title: Structural basis of lenacapavir-induced HIV-1 capsid disruption during virion maturation

    doi: 10.64898/2026.02.16.706074

    Figure Lengend Snippet: a , Overview of intra- and interhexamer interfaces within the HIV-1 capsid. b , Comparison of the CTD–CTD dimer interface between CA molecules mediated by helix 9, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the dimeric interface. Structures were superimposed by aligning one helix 9 of the CA-CTD of hexamer 1. c , Comparison of the CTD–CTD trimer interface between CA molecules mediated by primary helix 10, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 10 of the CA-CTD of hexamer 1. d , Comparison of the NTD–NTD interface between CA molecules mediated by helices 2 and 3, based on the cryo-EM structure of the LEN-bound CA lattice and the cryo-ET structure of a native CA lattice (EMDB: 13423). The LEN-bound CA lattice (blue) was superimposed onto the native CA lattice (yellow) to visualize structural differences at the trimeric interface. Structures were superimposed by aligning one helix 3 of the CA-NTD.

    Article Snippet: We then determined the capsid lattice structure within VLPs produced in the presence of LEN using a single-particle cryo-EM approach ( , Figs. S2-3, Table S1).

    Techniques: Comparison, Cryo-EM Sample Prep, Tomography